Read e-book online Affinity Chromatography. Biospecific Sorption–The First PDF

By O. Hoffmann-Ostenhof

ISBN-10: 0080226329

ISBN-13: 9780080226323

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Extra resources for Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry

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B i o c h e m . B i o p h y s . 154, 185 (1973). (13) D . B . C r a v e n , M . J . H a r v e y , C . R . Lowe & P . D . G . D e a n , Affinity c h r o m a t o g r a p h y on i m m o b i l i s e d a d e n o s i n e 5 ' - m o n o p h o s p h a t e 1. A new s y n t h e s i s and s o m e p r o p e r t i e s of an N - i m m o b i l i s e d 5 ' - A M P , E u r . J . B i o c h e m . 4 1 , 329 (1974). (14) R . L a m e d , Y. L e v i n & M . W i l c h e k , C o v a l e n t coupling of n u c l e o t i d e s to a g a r o s e for affinity c h r o m a t o g r a p h y , B i o c h i m .

Oil· c· 02 ra 0-2 (K Capacity 0-6 /o Fig· 5· The variation of capacity with the K A (apparent) for various immobilised nucleotides. The enzyme studied was yeast 6-phosphogluconate dehydrogenase. The ligands used were: a) N6-(3-aminopropylcarbamoylmethyl)-NADPi; b ) N 6 -(6-aminohexyl)5 f -AMP; c) ribose-immobilised NADP* (modular solid state synthesis); d) N°-(6-aminohexylcarbamoylmethyl)-2«-AMP; e) 8-(6-aminohexyl)amino-2f-AMP; f) N6-(-6-aminohexylcarbamoylmethyl)-NADP ; g) ribose-immobilized-NADP+.

J. 135, 595 (1973)· 13. Schmidt, H. L. and Grenner, G. Coenzyme properties of NAD bound to different matrices through the amino group in the 6-position, Eur. J. Biochem. 67, 295 (1976). 37 38 Peter D. G. Dean and David H. Watson 1^-. -O. and Mosbach, K. The synthesis of Three AMP"Analogues and their Application as General Ligands in Biospecific Affinity Chromatography, Eur. J. Biochem. ^ 7 , 8l (197*0· 15· Trayer, I. P. and Trayer, H. Affinity Chromatography of Nicotinamide Nucleotide~Dependent Dehydrogenases on Immobilised Nucleotide Derivatives, Biochem.

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Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry by O. Hoffmann-Ostenhof


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